IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/09/10: Difference between revisions
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== | ==9/10== | ||
* | <u>Ethanol</u><br> | ||
Resting Cell Assay started at 8:00 | |||
*O.D. of cells was adjusted to be approx. 1.0 (+-0.2) | |||
*Cells were resuspended in 30 ml PBS | |||
*3 ml of 20% glucose and 1 ml of 90 g/L Pyruvate (equivelant of .09 g per 1 ml, which, when diluted in 30 ml is equal to 3 g/L) was then added. | |||
*Samples were taken every 2 hours for 8 hours, and then a 24 hour sample was taken. | |||
Latest revision as of 00:16, 27 September 2017
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9/10Ethanol
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