IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/09/10: Difference between revisions

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(Autocreate 2014/09/10 Entry for IGEM:Arizona_State/2014/Notebook/asu_igem_2014)
 
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==Entry title==
==9/10==
* Insert content here...
<u>Ethanol</u><br>
Resting Cell Assay started at 8:00
*O.D. of cells was adjusted to be approx. 1.0 (+-0.2)
*Cells were resuspended in 30 ml PBS
*3 ml of 20% glucose and 1 ml of 90 g/L Pyruvate (equivelant of .09 g per 1 ml, which, when diluted in 30 ml is equal to 3 g/L) was then added.
*Samples were taken every 2 hours for 8 hours, and then a 24 hour sample was taken.  





Revision as of 18:09, 10 September 2014

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9/10

Ethanol
Resting Cell Assay started at 8:00

  • O.D. of cells was adjusted to be approx. 1.0 (+-0.2)
  • Cells were resuspended in 30 ml PBS
  • 3 ml of 20% glucose and 1 ml of 90 g/L Pyruvate (equivelant of .09 g per 1 ml, which, when diluted in 30 ml is equal to 3 g/L) was then added.
  • Samples were taken every 2 hours for 8 hours, and then a 24 hour sample was taken.