IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/09/10

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

9/10

Ethanol
Resting Cell Assay started at 8:00

  • O.D. of cells was adjusted to be approx. 1.0 (+-0.2)
  • Cells were resuspended in 30 ml PBS
  • 3 ml of 20% glucose and 1 ml of 90 g/L Pyruvate (equivelant of .09 g per 1 ml, which, when diluted in 30 ml is equal to 3 g/L) was then added.
  • Samples were taken every 2 hours for 8 hours, and then a 24 hour sample was taken.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Arizona_State/2014/Notebook/asu_igem_2014/2014/09/10&oldid=819481"