IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/09/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==SOEing gel== | ||
> SOEing gel | |||
{| class="wikitable" border="0" cellspacing="3" <!-- PCR rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> | |||
| rowspan="7" | <u>Expected:</u><br>1. Amplicon 1 = 746<br>2. Amplicon 2 = 170<br> 3. Final Product = 916<br> | |||
| rowspan="7" | [[Image:20141001 111800 SOEing gel.jpg|500px|PCR 10/1/14]]<br>10 μL/lane; 1% agarose | |||
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| DNA(accD) || 1.0 | |||
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| 10 μM primer 1 || 1.0 | |||
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| 10 μM primer 2 || 1.0 | |||
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| 2x GoTaq mix|| 25.0 | |||
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| dH<sub>2</sub>O || 22.0 | |||
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| || 50 μL | |||
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Latest revision as of 00:20, 27 September 2017
Project name | Main project page Previous entry | ||||||||||||||||
SOEing gel> SOEing gel
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