IGEM:Arizona State/2014/Notebook/asu igem 2014/2014/09/17: Difference between revisions

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| <u>Reagent</u> || <u>Volume</u>  
| <u>Reagent</u> || <u>Volume</u>  
| rowspan="7" | <u>Expected:</u><br>1. Amplicon 1 = 746<br>2. Amplicon 2 = 170<br> 3. Final Product = 916<br>
| rowspan="7" | <u>Expected:</u><br>1. Amplicon 1 = 746<br>2. Amplicon 2 = 170<br> 3. Final Product = 916<br>
| rowspan="7" | [[Image:20141001 111800 SOEing gel.jpg|1000px|PCR 10/1/14]]<br>10 μL/lane; 1% agarose
| rowspan="7" | [[Image:20141001 111800 SOEing gel.jpg|500px|PCR 10/1/14]]<br>10 μL/lane; 1% agarose
|-
|-
| DNA(accD) || 1.0
| DNA(accD) || 1.0

Revision as of 20:19, 23 September 2014

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SOEing gel

> SOEing gel

Reagent Volume Expected:
1. Amplicon 1 = 746
2. Amplicon 2 = 170
3. Final Product = 916
 PCR 10/1/14
10 μL/lane; 1% agarose
DNA(accD) 1.0
10 μM primer 1 1.0
10 μM primer 2 1.0
2x GoTaq mix 25.0
dH2O 22.0
  50 μL


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