IGEM:Berkeley/2010: Difference between revisions

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'''Procedures'''
'''Procedures'''


===Polymerase Reactions===
''Polymerase Reactions''
[[Template:SBB-Protocols_PCR1 | Wobble Reaction]]<br>
[[Template:SBB-Protocols_PCR1 | Wobble Reaction]]<br>
[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br>
[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br>
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[[Template:SBB-Protocols_QuickChange | QuikChange]]<br>
[[Template:SBB-Protocols_QuickChange | QuikChange]]<br>


===Cleanup Reactions===
''Cleanup Reactions''
[[Template:SBB-Protocols_Zymo1 | Regular Zymo Cleanup]]<br>
[[Template:SBB-Protocols_Zymo1 | Regular Zymo Cleanup]]<br>
[[Template:SBB-Protocols_Zymo2 | Small-Frag Zymo Cleanup]]<br>
[[Template:SBB-Protocols_Zymo2 | Small-Frag Zymo Cleanup]]<br>
[[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification]]<br>
[[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification]]<br>


===Digestions Reactions===
''Digestions Reactions''
[[Template:SBB-Protocols_Enz1 | EcoRI/BamHI Digest of Wobble Products]]<br>
[[Template:SBB-Protocols_Enz1 | EcoRI/BamHI Digest of Wobble Products]]<br>
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br>
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br>
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[[Template:SBB-Protocols_Enz6 | Analytical digests (Mapping)]]<br>
[[Template:SBB-Protocols_Enz6 | Analytical digests (Mapping)]]<br>


===Bacteria Manipulations===
''Bacteria Manipulations''
[[Template:SBB-Protocols_Micro1 | Transformation by heat-shock]]<br>
[[Template:SBB-Protocols_Micro1 | Transformation by heat-shock]]<br>
[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br>
[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br>
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br>
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br>
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br>
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br>
===Assays===


 
''Other''
===Other===
[[Template:SBB-Protocols_LRGtw | LR Gateway Transfers]]<br>
[[Template:SBB-Protocols_LRGtw | LR Gateway Transfers]]<br>
[[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br>
[[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br>
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'''Subgroup Notebooks'''
'''Subgroup Notebooks'''


[[Berk2006-ConjugationTeam | Conjugation 6/7/06-7/21/06]]<br>
 
[[Berk2006-ConjugationTeam2 | Conjugation 7/24/06 - 09/07/06]]<br>
[[Berk2006-ConjugationTeam3 | Conjugation 09/07/06- present]]<br>
[[Berk2006-LocksAndKeysTeam | Locks and Keys]]<br>
[[Berk2006-LogicGatesTeam | Logic Gates]]<br>
[http://www.openwetware.org/wiki/IGEM:UC_Berkeley/2006/bryans_notebook  Bryan's Lock and Keys]<br>
[[Berk2006-Samantha's Notebook | Samantha's Notebook]]<br>


<br>
<br>

Revision as of 13:43, 4 June 2010

Berkeley iGEM 2010

Gel Pictures


Project Name

Project Summary


<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Team


Undergrads
Amy Kristofferson
Conor McClune
Tahoura
Daniela
Christoph

Graduate Students
Tim Hsiau

Postdocs

Insert names here

Advisors
Chris Anderson
Terry Johnson

<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>Tools


Lab calendar

The Details

Oligonucleotides
Sequences
Construction Files
-80 Stocks
Sequencing

Procedures

Polymerase Reactions Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange

Cleanup Reactions Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep

Other LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA


Subgroup Notebooks



<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Project


Addressable Cell-to-Cell Communication

Useful Links


Subgroup Strategies, Overview