IGEM:Berkeley/2010: Difference between revisions
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[[Gel Pictures]] | <hr class=divider> | ||
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| rowspan=2 | [[Image:berkeleyCampanile01.jpg|left|200px]]'''Choa Choa's Delivery Service''' | |||
The ability to manipulate the DNA of an organism is vital to many modern fields of biology. While we have perfected this in common research species such as E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting to researchers because they are the closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe who study these little creatures are hindered by the inability to genetically manipulate them. | |||
The Berkeley iGEM 2010 team is applying synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it is programmed to burst using a self-lysis device. Proteins we have placed inside the bacteria will then go into action. First, we have designed a vacuole-buster device that will burst the small food membrane holding the bacteria inside the choano, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nucleus, the transposase will splice the transposon into the choanoflagellate DNA. | |||
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<img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> | |||
</html>The Team</h3> | |||
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[[Image:Teamberk10.jpg | 200px]] | |||
'''Undergrads'''<br> | |||
[http://openwetware.org/wiki/User:Amy_N._Kristofferson Amy Kristofferson] <br> | |||
[[Conor McClune]] <br> | |||
[http://openwetware.org/wiki/User:Tahoura_Samad Tahoura Samad] <br> | |||
[http://openwetware.org/wiki/User:Daniela_Mehech Daniela] <br> | |||
[http://openwetware.org/wiki/User:Christoph_Neyer Christoph Neyer] <br> | |||
'''Graduate Students'''<br> | |||
[http://www.epernicus.com/th2 Tim Hsiau]<br> | |||
'''Postdocs'''<br> | |||
Insert names here | |||
'''Advisors'''<br> | |||
[[Chris Anderson]] <br> | |||
[http://www.openwetware.org/wiki/PP_-_Terry Terry Johnson] <br> | |||
[[Image:Ladder pic.png]] | |||
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<img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> | |||
</html>Tools</h3> | |||
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'''Extremely Important Material'''<br> | |||
[[Quotes]] <br> | |||
[[Nicknames]] <br> | |||
'''Lab Notebooks''' | |||
[[Berk2010-Group | Group]] <br> | |||
[[Berk2010-Amy | Amy]]<br> | |||
[[Berk2010-Conor | Conor]]<br> | |||
[[Berk2010-Daniela | Daniela]] <br> | |||
[[Berk2010-Tahoura | Tahoura]]<br> | |||
[[Berk2010-Christoph | Christoph]]<br> | |||
[[Berk2010-Tim | Tim]]<br> | |||
'''The Details''' | |||
[https://spreadsheets1.google.com/ccc?key=tCL7NVstt-4T_wcRbwe-kWg&hl=en#gid=8 Oligonucleotides]<br> | |||
[[Berk2010-Gel Pictures | Gel Pictures]]<br> | |||
[[Berk2010-Sequencing | Sequencing]]<br> | |||
[[Berk2010-Constructs | Construction Files]]<br> | |||
[https://spreadsheets1.google.com/ccc?key=tCL7NVstt-4T_wcRbwe-kWg&hl=en#gid=13 -80 Stocks]<br> | |||
[[Berk2010-Assembly | Assembly Trees]]<br> | |||
[[Berk2010-New Composite Parts | New Composite Parts]]<br> | |||
[[Berk2010-Robot CSV Files | Robot CSV Files]]<br> | |||
'''Procedures''' | |||
''Polymerase Reactions''<br> | |||
[[Template:SBB-Protocols_PCR1 | Wobble Reaction]]<br> | |||
[[Template:SBB-Protocols_PCR2 | Cloning by PCR]]<br> | |||
[[Template:SBB-Protocols_PCR3 | SOEing PCR]]<br> | |||
[[Template:SBB-PCA | PCA Gene Synthesis]]<br> | |||
[[Template:SBB-Protocols_QuickChange | QuikChange]]<br> | |||
[[Template:SSB-Protocols_Col_PCR | Colony PCR]] <br> | |||
''Cleanup Reactions''<br> | |||
[[Template:SBB-Protocols_Zymo1 | Regular Zymo Cleanup]]<br> | |||
[[Template:SBB-Protocols_Zymo2 | Small-Frag Zymo Cleanup]]<br> | |||
[[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification]]<br> | |||
''Digestions Reactions''<br> | |||
[[Template:SBB-Protocols_Enz1 | EcoRI/BamHI Digest of Wobble Products]]<br> | |||
[[Template:SBB-Protocols_Enz2 | EcoRI/BamHI Digest of PCR Products]]<br> | |||
[[Manual 2ab Assembly Digest]] <br> | |||
[[Template:SBB-Protocols_Enz3 | BglII Digest of EIPCR Products]]<br> | |||
[[Template:SBB-Protocols_Enz4 | Ligation of EcoRI/BamHI digests]]<br> | |||
[[Template:SBB-Protocols_Enz5 | Ligation of EIPCR digests]]<br> | |||
[[Template:SBB-Protocols_Enz6 | Analytical digests (Mapping)]]<br> | |||
''Bacteria Manipulations''<br> | |||
[[Template:SBB-Protocols_Micro1 | Transformation by heat-shock]]<br> | |||
[[Template:SBB-Protocols_Micro2 | Picking of colonies]]<br> | |||
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | |||
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br> | |||
[[Template:8-Strip Machery Nagel Miniprep | 8-Strip Machery Nagel Miniprep]] <br> | |||
[[Genomic Miniprep]]<br> | |||
[[-80 Glycerol Stocks]]<br> | |||
[[Making Competent Cells]]<br> | |||
[[Small Scale Electrocompetent Prep-Generic ]]<br> | |||
''Other''<br> | |||
[[Template:SBB-Protocols_LRGtw | LR Gateway Transfers]]<br> | |||
[[Template:SBB-EcoBamXfers | EcoRI/BamHI Part Transfers]]<br> | |||
[[Template:SBB-Protocols_SeqResult | What do I do with my sequencing data?]]<br> | |||
[[Template:SBB-Protocols_SmallScaleCompetent | Small Scale Competent Cell Transformation]]<br> | |||
[[Template:SBB-Protocols_ELISA | ELISA]]<br> | |||
[[2ab Assembly]]<br> | |||
[[Gibson Assembly]]<br> | |||
[[Choano Food Preperation]] | |||
'''Subgroup Notebooks''' | |||
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<h3><html> | |||
<img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> | |||
</html>Project</h3> | |||
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'''Useful Links''' | |||
'''Subgroup Strategies, Overview''' | |||
[http://2010.igem.org/Team:Berkeley '''iGEM Wiki for Berkeley'''] | |||
|} | |||
Latest revision as of 12:29, 2 December 2010
 The ability to manipulate the DNA of an organism is vital to many modern fields of biology. While we have perfected this in common research species such as E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting to researchers because they are the closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe who study these little creatures are hindered by the inability to genetically manipulate them. The Berkeley iGEM 2010 team is applying synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it is programmed to burst using a self-lysis device. Proteins we have placed inside the bacteria will then go into action. First, we have designed a vacuole-buster device that will burst the small food membrane holding the bacteria inside the choano, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nucleus, the transposase will splice the transposon into the choanoflagellate DNA. |
