IGEM:Berkeley/2010: Difference between revisions
No edit summary |
JCAnderson (talk | contribs) No edit summary |
||
(6 intermediate revisions by 3 users not shown) | |||
Line 4: | Line 4: | ||
<div id="about"> | <div id="about"> | ||
{| | {| | ||
| rowspan=2 | [[Image:berkeleyCampanile01.jpg|left|200px]]''' | | rowspan=2 | [[Image:berkeleyCampanile01.jpg|left|200px]]'''Choa Choa's Delivery Service''' | ||
The ability to manipulate the DNA of an organism is vital to many modern fields of biology. | The ability to manipulate the DNA of an organism is vital to many modern fields of biology. While we have perfected this in common research species such as E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting to researchers because they are the closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe who study these little creatures are hindered by the inability to genetically manipulate them. | ||
The Berkeley iGEM 2010 team is | The Berkeley iGEM 2010 team is applying synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it is programmed to burst using a self-lysis device. Proteins we have placed inside the bacteria will then go into action. First, we have designed a vacuole-buster device that will burst the small food membrane holding the bacteria inside the choano, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nucleus, the transposase will splice the transposon into the choanoflagellate DNA. | ||
|- | |- | ||
Line 113: | Line 113: | ||
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | [[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br> | ||
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br> | [[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br> | ||
[[Template:8-Strip Machery Nagel Miniprep | 8-Strip Machery Nagel Miniprep]] <br> | |||
[[Genomic Miniprep]]<br> | [[Genomic Miniprep]]<br> | ||
[[-80 Glycerol Stocks]]<br> | [[-80 Glycerol Stocks]]<br> | ||
Line 125: | Line 126: | ||
[[Template:SBB-Protocols_ELISA | ELISA]]<br> | [[Template:SBB-Protocols_ELISA | ELISA]]<br> | ||
[[2ab Assembly]]<br> | [[2ab Assembly]]<br> | ||
[[Gibson Assembly]] | [[Gibson Assembly]]<br> | ||
[[Choano Food Preperation]] | |||
Line 148: | Line 150: | ||
'''Subgroup Strategies, Overview''' | '''Subgroup Strategies, Overview''' | ||
[http://2010.igem.org/Team:Berkeley '''iGEM Wiki for Berkeley'''] | |||
|} | |} |
Latest revision as of 12:29, 2 December 2010
Choa Choa's Delivery Service
The ability to manipulate the DNA of an organism is vital to many modern fields of biology. While we have perfected this in common research species such as E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting to researchers because they are the closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe who study these little creatures are hindered by the inability to genetically manipulate them. The Berkeley iGEM 2010 team is applying synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it is programmed to burst using a self-lysis device. Proteins we have placed inside the bacteria will then go into action. First, we have designed a vacuole-buster device that will burst the small food membrane holding the bacteria inside the choano, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nucleus, the transposase will splice the transposon into the choanoflagellate DNA. |