IGEM:Berkeley/2010: Difference between revisions

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[[Template:SBB-Protocols_ELISA | ELISA]]<br>
[[Template:SBB-Protocols_ELISA | ELISA]]<br>
[[2ab Assembly]]<br>
[[2ab Assembly]]<br>
[[-80 Glycerol Stocks]]
[[-80 Glycerol Stocks]]<br>
[[Making Competent Cells]]<br>




Revision as of 10:54, 9 June 2010

Project Name

Project Summary

<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>The Team

Undergrads

Amy Kristofferson
Conor McClune
Tahoura
Daniela
Christoph Neyer

Graduate Students
Tim Hsiau

Postdocs

Insert names here

Advisors
Chris Anderson
Terry Johnson


<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>Tools

Lab calendar

Lab Notebooks

Group
Amy
Conor
Daniela
Tahoura
Christoph
Tim
The Details

Oligonucleotides
Gel Pictures
Sequencing
Construction Files
-80 Stocks
Assembly Trees
New Composite Parts



Procedures

Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange
Colony PCR

Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep

Other
LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA
2ab Assembly
-80 Glycerol Stocks
Making Competent Cells


Subgroup Notebooks



<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Project


Useful Links


Subgroup Strategies, Overview

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