IGEM:Berkeley/2010: Difference between revisions

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[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br>
[[Template:SBB-Protocols_Micro3 | Miniprep purification of DNA]]<br>
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br>
[[Template:SBB-Protocols_Micro4 | Macherey-Nagel Miniprep]]<br>
[Template:SBB-Protocols_Micro2 | Genomic Miniprep]]<br>
[Template:SBB-Protocols_Micro5| Genomic Miniprep]]<br>
[[-80 Glycerol Stocks]]<br>
[[-80 Glycerol Stocks]]<br>
[[Making Competent Cells]]<br>
[[Making Competent Cells]]<br>

Revision as of 17:13, 15 June 2010

Project Name

Project Summary

<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>The Team

Undergrads

Amy Kristofferson
Conor McClune
Tahoura Samad
Daniela
Christoph Neyer

Graduate Students
Tim Hsiau

Postdocs

Insert names here

Advisors
Chris Anderson
Terry Johnson


<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>Tools


Extremely Important Material
Quotes
Nicknames


Lab Notebooks

Group
Amy
Conor
Daniela
Tahoura
Christoph
Tim


The Details

Oligonucleotides
Gel Pictures
Sequencing
Construction Files
-80 Stocks
Assembly Trees
New Composite Parts
Robot CSV Files


Procedures

Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange
Colony PCR

Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
Manual 2ab Assembly Digest
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep
[Template:SBB-Protocols_Micro5| Genomic Miniprep]]
-80 Glycerol Stocks
Making Competent Cells

Other
LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA
2ab Assembly


Subgroup Notebooks



<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Resources"> </html>Project


Useful Links


Subgroup Strategies, Overview

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