IGEM:Berkeley/2010 - Revision history

JCAnderson at 19:29, 2 December 2010

JCAnderson — Thu, 02 Dec 2010 19:29:06 GMT

←Older revision		Revision as of 19:29, 2 December 2010
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	'''Subgroup Strategies, Overview'''		'''Subgroup Strategies, Overview'''
		+
		+
		+	[http://2010.igem.org/Team:Berkeley '''iGEM Wiki for Berkeley''']

	\|}		\|}

Conor McClune at 06:47, 27 October 2010

Conor McClune — Wed, 27 Oct 2010 06:47:19 GMT

←Older revision		Revision as of 06:47, 27 October 2010
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	<div id="about">		<div id="about">
	{\|		{\|
-	\| rowspan=2 \| [[Image:berkeleyCampanile01.jpg\|left\|200px]]'''~~Project Name~~'''	+	\| rowspan=2 \| [[Image:berkeleyCampanile01.jpg\|left\|200px]]'''Choa Choa's Delivery Service'''

	The ability to manipulate the DNA of an organism is vital to many modern fields of biology. While we have perfected this in common research species such as E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting to researchers because they are the closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe who study these little creatures are hindered by the inability to genetically manipulate them.		The ability to manipulate the DNA of an organism is vital to many modern fields of biology. While we have perfected this in common research species such as E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting to researchers because they are the closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe who study these little creatures are hindered by the inability to genetically manipulate them.

Conor McClune at 23:21, 3 September 2010

Conor McClune — Fri, 03 Sep 2010 23:21:51 GMT

←Older revision		Revision as of 23:21, 3 September 2010
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	[[Template:SBB-Protocols_ELISA \| ELISA]]<br>		[[Template:SBB-Protocols_ELISA \| ELISA]]<br>
	[[2ab Assembly]]<br>		[[2ab Assembly]]<br>
-	[[Gibson Assembly]]	+	[[Gibson Assembly]]<br>
	[[Choano Food Preperation]]		[[Choano Food Preperation]]

Conor McClune at 23:21, 3 September 2010

Conor McClune — Fri, 03 Sep 2010 23:21:35 GMT

←Older revision		Revision as of 23:21, 3 September 2010
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	[[2ab Assembly]]<br>		[[2ab Assembly]]<br>
	[[Gibson Assembly]]		[[Gibson Assembly]]
		+	[[Choano Food Preperation]]

Daniela Mehech at 14:50, 22 July 2010

Daniela Mehech — Thu, 22 Jul 2010 14:50:55 GMT

←Older revision		Revision as of 14:50, 22 July 2010
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	{\|		{\|
	\| rowspan=2 \| [[Image:berkeleyCampanile01.jpg\|left\|200px]]'''Project Name'''		\| rowspan=2 \| [[Image:berkeleyCampanile01.jpg\|left\|200px]]'''Project Name'''
-	~~Project Summary <br>~~	+
-	The ability to manipulate the DNA of an organism is vital to many modern fields of biology. ~~However, while~~ we have perfected this in common research species ~~like~~ E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting because they closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe study these little creatures~~, but~~ are hindered by an inability to genetically manipulate them.	+	The ability to manipulate the DNA of an organism is vital to many modern fields of biology. While we have perfected this in common research species such as E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting to researchers because they are the closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe who study these little creatures are hindered by the inability to genetically manipulate them.

-	The Berkeley iGEM 2010 team is ~~trying to apply~~ synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it ~~will be~~ programmed to burst using a self-lysis device. ~~Then, proteins~~ we have placed inside the bacteria will go into action. First, we have designed a vacuole-buster device that will burst the small food membrane inside the choano ~~that held the bacteria~~, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nucleus, the transposase will splice the transposon into the choanoflagellate DNA.	+	The Berkeley iGEM 2010 team is applying synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it is programmed to burst using a self-lysis device. Proteins we have placed inside the bacteria will then go into action. First, we have designed a vacuole-buster device that will burst the small food membrane holding the bacteria inside the choano, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nucleus, the transposase will splice the transposon into the choanoflagellate DNA.
	\|-		\|-

Christoph Neyer at 22:28, 14 July 2010

Christoph Neyer — Wed, 14 Jul 2010 22:28:52 GMT

←Older revision		Revision as of 22:28, 14 July 2010
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	[[Template:SBB-Protocols_Micro3 \| Miniprep purification of DNA]]<br>		[[Template:SBB-Protocols_Micro3 \| Miniprep purification of DNA]]<br>
	[[Template:SBB-Protocols_Micro4 \| Macherey-Nagel Miniprep]]<br>		[[Template:SBB-Protocols_Micro4 \| Macherey-Nagel Miniprep]]<br>
		+	[[Template:8-Strip Machery Nagel Miniprep \| 8-Strip Machery Nagel Miniprep]] <br>
	[[Genomic Miniprep]]<br>		[[Genomic Miniprep]]<br>
	[[-80 Glycerol Stocks]]<br>		[[-80 Glycerol Stocks]]<br>

Conor McClune at 23:53, 2 July 2010

Conor McClune — Fri, 02 Jul 2010 23:53:14 GMT

←Older revision		Revision as of 23:53, 2 July 2010
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	The ability to manipulate the DNA of an organism is vital to many modern fields of biology. However, while we have perfected this in common research species like E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting because they closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe study these little creatures, but are hindered by an inability to genetically manipulate them.		The ability to manipulate the DNA of an organism is vital to many modern fields of biology. However, while we have perfected this in common research species like E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting because they closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe study these little creatures, but are hindered by an inability to genetically manipulate them.

-	The Berkeley iGEM 2010 team is trying to apply synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it will be programmed to burst using a self-lysis device. Then, proteins we have placed inside the bacteria will go into action. First, we have designed a vacuole-buster device that will burst the small food membrane inside the choano that held the bacteria, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the ~~nuclous~~, the transposase will splice the transposon into the choanoflagellate DNA.	+	The Berkeley iGEM 2010 team is trying to apply synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it will be programmed to burst using a self-lysis device. Then, proteins we have placed inside the bacteria will go into action. First, we have designed a vacuole-buster device that will burst the small food membrane inside the choano that held the bacteria, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nucleus, the transposase will splice the transposon into the choanoflagellate DNA.
	\|-		\|-

Conor McClune at 21:52, 2 July 2010

Conor McClune — Fri, 02 Jul 2010 21:52:11 GMT

←Older revision		Revision as of 21:52, 2 July 2010
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	\| rowspan=2 \| [[Image:berkeleyCampanile01.jpg\|left\|200px]]'''Project Name'''		\| rowspan=2 \| [[Image:berkeleyCampanile01.jpg\|left\|200px]]'''Project Name'''
	Project Summary <br>		Project Summary <br>
		+	The ability to manipulate the DNA of an organism is vital to many modern fields of biology. However, while we have perfected this in common research species like E. coli, yeast and mouse cells, it is still impossible to transform many other species researchers study. Our project is an attempt to develop transgenics ) techniques for a family of single celled organisms called choanoflagellates. These species are interesting because they closest living relative to our microbial ancestor that became the first multicellular animal. Nicole King, here at UC Berkeley, and other researchers across the globe study these little creatures, but are hindered by an inability to genetically manipulate them.

		+	The Berkeley iGEM 2010 team is trying to apply synthetic biology to this problem. We are engineering bacteria that can deliver DNA into the choano. Choanos are predatory, which makes our job a bit simpler. Once our bacteria is engulfed by the choano, it will be programmed to burst using a self-lysis device. Then, proteins we have placed inside the bacteria will go into action. First, we have designed a vacuole-buster device that will burst the small food membrane inside the choano that held the bacteria, spewing the contents into the cytoplasm of the cell. In the cytoplasm, a transposon/transposase device tagged with a nuclear localization device will move to the nucleus. In the nuclous, the transposase will splice the transposon into the choanoflagellate DNA.
	\|-		\|-

Christoph Neyer at 21:34, 21 June 2010

Christoph Neyer — Mon, 21 Jun 2010 21:34:14 GMT

←Older revision		Revision as of 21:34, 21 June 2010
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	[[Chris Anderson]] <br>		[[Chris Anderson]] <br>
	[http://www.openwetware.org/wiki/PP_-_Terry Terry Johnson] <br>		[http://www.openwetware.org/wiki/PP_-_Terry Terry Johnson] <br>
		+	[[Image:Ladder pic.png]]

Conor McClune at 01:52, 21 June 2010

Conor McClune — Mon, 21 Jun 2010 01:52:41 GMT

←Older revision		Revision as of 01:52, 21 June 2010
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	[[Template:SBB-Protocols_SmallScaleCompetent \| Small Scale Competent Cell Transformation]]<br>		[[Template:SBB-Protocols_SmallScaleCompetent \| Small Scale Competent Cell Transformation]]<br>
	[[Template:SBB-Protocols_ELISA \| ELISA]]<br>		[[Template:SBB-Protocols_ELISA \| ELISA]]<br>
-	[[2ab Assembly]]	+	[[2ab Assembly]]<br>
		+	[[Gibson Assembly]]