IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/01: Difference between revisions

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* New plates will be made in the afternoon.
* New plates will be made in the afternoon.


 
==Testing of Chloramphenicol at 100 µg/mL Concentration==
* Used two LB-agar plates
** One for LB-only control
** One for LB + Chlor-100 test
* Plated 80 µL of 25 mg/mL chloramphenicol onto 20 mL LB agar plate for a final concentration of 100 µg/mL
* Let dry for 1 hour.


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Revision as of 12:13, 1 June 2009

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Results of Chloramphenicol Testing

  • Chloramphenicol plates did not work.
  • Growth was observed on both the LB-only and LB+Chloramphenicol plates.
  • Both DB3.1 and DH5alpha cells grew on both sets of plates.
  • Could possibly be an issue with the concentration. See here.
    • This page states that chloramphenicol plates should be made at 100 µg/mL concentration rather than 25 µg/mL.
    • To do this, we would need to plate 80 µL on each LB plate rather than 20 µL.
  • New plates will be made in the afternoon.

Testing of Chloramphenicol at 100 µg/mL Concentration

  • Used two LB-agar plates
    • One for LB-only control
    • One for LB + Chlor-100 test
  • Plated 80 µL of 25 mg/mL chloramphenicol onto 20 mL LB agar plate for a final concentration of 100 µg/mL
  • Let dry for 1 hour.


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