IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/03: Difference between revisions

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** Plates were left on Roza's bench at 10:26 a.m.
** Plates were left on Roza's bench at 10:26 a.m.


==Preparation of LB-Agar Plates
==Preparation of LB-Agar Plates==
* In anticipation of the need for more LB-Agar, I will make more LB-Agar and ask someone to pour the plates this afternoon when it is autoclaved.
* In anticipation of the need for more LB-Agar, I will make more LB-Agar and ask someone to pour the plates this afternoon when it is autoclaved.
* LB Agar composition was:
* LB Agar composition was:
** 13g of LB-Agar in 350 mL of sdH2O.
** 13g of LB-Agar in 350 mL of sdH2O.
** Prepared two bottles.
** Prepared two bottles.
==Chloramphenicol Testing for Paul's new batch==
* Paul brought over a new batch of chloramphenicol from his lab for us to use.
* Stock was made at 25 mg/mL in 100% MeOH.
* Steps taken to test chloramphenicol:
** Spread plated 25 µL of new chloramphenicol onto 1 LB agar plate (Chlor 1). LB-only is the control (Chlor 0)
** Let dry for 10 minutes.
** Streaked DB3.1 and DH5a onto each of the plates as done previously.
** Stored at Lagally Lab 37ºC incubator at 11:43 AM.
** Paul's chloramphenicol stored at Lagally Lab -20ºC freezer; will make aliquots of them after confirmation of efficacy.


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Revision as of 15:49, 15 June 2009

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Chloramphenicol Testing Results

  • This is a continuation of previous day's test.
  • Took plates out at 10:15 am.
  • Results are as follows:
Plate Growth for DB3.1 Growth for DH5a
Chlor 1 Yes Yes
Chlor 2 Yes Yes
Chlor 3 No No
  • Conclusions:
    • Our batch of chloramphenicol must be faulty, or we are preparing it the wrong way. It may also be the wrong form of Chlor.
    • For now, I will call Paul to see if we can borrow his lab's chloramphenicol.
    • Plates were left on Roza's bench at 10:26 a.m.

Preparation of LB-Agar Plates

  • In anticipation of the need for more LB-Agar, I will make more LB-Agar and ask someone to pour the plates this afternoon when it is autoclaved.
  • LB Agar composition was:
    • 13g of LB-Agar in 350 mL of sdH2O.
    • Prepared two bottles.

Chloramphenicol Testing for Paul's new batch

  • Paul brought over a new batch of chloramphenicol from his lab for us to use.
  • Stock was made at 25 mg/mL in 100% MeOH.
  • Steps taken to test chloramphenicol:
    • Spread plated 25 µL of new chloramphenicol onto 1 LB agar plate (Chlor 1). LB-only is the control (Chlor 0)
    • Let dry for 10 minutes.
    • Streaked DB3.1 and DH5a onto each of the plates as done previously.
    • Stored at Lagally Lab 37ºC incubator at 11:43 AM.
    • Paul's chloramphenicol stored at Lagally Lab -20ºC freezer; will make aliquots of them after confirmation of efficacy.



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