IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/03: Difference between revisions
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* HyClone DPBS/Modified (-Calcium, -Magnesium) from Thermo Scientific was pre-chilled in ice water bath. | * HyClone DPBS/Modified (-Calcium, -Magnesium) from Thermo Scientific was pre-chilled in ice water bath. | ||
* Prepared test tubes with 9 mL or 9.9 mL of LB (18 tubes each) | * Prepared test tubes with 9 mL or 9.9 mL of LB (18 tubes each) | ||
* Prepared two 96-well dilution plates with either 900µL or 990µL/well for dilutions | |||
* Measured OD of each sample | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Strain''' | |||
| align="center" style="background:#f0f0f0;"|'''Dilution''' | |||
| align="center" style="background:#f0f0f0;"|'''OD600''' | |||
| align="center" style="background:#f0f0f0;"|'''Time Measured''' | |||
| align="center" style="background:#f0f0f0;"|'''Actual OD660''' | |||
| align="center" style="background:#f0f0f0;"|'''Bacterial V1 (mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''To-Up''' | |||
|- | |||
| BW27783||1 in 20||0.663||2:50 PM||13.26||0.49||49.5 | |||
|- | |||
| DB3.1||1 in 20||0.384||2:52 PM||7.68||0.85||49.2 | |||
|- | |||
| DH5a||1 in 10||0.801||2:53 PM||8.01||0.81||49.2 | |||
|} | |||
* Put 50 mL of bacteria in each flask. Remeasured OD600: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Strain''' | |||
| align="center" style="background:#f0f0f0;"|'''OD600''' | |||
| align="center" style="background:#f0f0f0;"|'''Time Measured''' | |||
|- | |||
| BW27783||0.103||3:09 PM | |||
|- | |||
| DB3.1||0.133||3:09 PM | |||
|- | |||
| DH5a||0.107||3:09 PM | |||
|} | |||
* Results for OD Readings | |||
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Revision as of 13:28, 7 August 2009
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Chloramphenicol Testing Results
Preparation of LB-Agar Plates
Chloramphenicol Testing for Paul's new batch
Growth Curve for E. coli DB3.1, DH5a and BW27783
Steps Taken
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