IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/03: Difference between revisions
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| DH5a||0.107||3:09 PM | | DH5a||0.107||3:09 PM | ||
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===Results for OD Readings=== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Time''' | |||
| align="center" style="background:#f0f0f0;"|'''Strain''' | |||
| align="center" style="background:#f0f0f0;"|'''OD600 (1)''' | |||
| align="center" style="background:#f0f0f0;"|'''OD600 (2)''' | |||
|- | |||
| 3:44 PM||BW27783||0.014||0.016 | |||
|- | |||
| 3:46 PM||DH5a||0.016||0.008 | |||
|- | |||
| 3:51 PM||DB3.1||0.092||0.086 | |||
|- | |||
| 4:18 PM||BW27783||0.085||0.084 | |||
|- | |||
| 4:21 PM||DH5a||0.135||0.149 | |||
|- | |||
| 4:22 PM||DB3.1||0.223||0.228 | |||
|- | |||
| 4:42 PM||BW27783||0.078||0.073 | |||
|- | |||
| 4:43 PM||DH5a||0.117||0.111 | |||
|- | |||
| 4:44 PM||DB3.1||0.259||0.244 | |||
|- | |||
| 5:08 PM||BW27783||0.070||0.075 | |||
|- | |||
| 5:09 PM||DH5a||0.095||0.095 | |||
|- | |||
| 5:10 PM||DB3.1||0.355||0.354 | |||
|} | |||
===Comments on Results=== | |||
# OD readings fluctuated throughout the timecourse, and this was not as expected. | |||
# When Calvin did his OD readings, he noticed that the blank (LB-only) read 0.2 when measured as a "sample" after measuring the prior samples. | |||
# This Friday, we will repeat the experiment with the following modifications: | |||
## No plating of bacteria - because we are not interested in the number of cells (too troublesome) | |||
## We will be using the Finlay Lab spectrophotometer to do the OD measurements. | |||
## The experiment will be started earlier at 9:00 AM to finish off earlier. | |||
# The plates that were plated today are placed into the AMBL 37ºC incubator at 6:30PM. They will be checked tomorrow. | |||
==Summary of the Day== | |||
# First batch of chloramphenicol from Paul's lab worked while ours didn't. | |||
# Paul made a new batch of chloramphenicol and they are being tested now. | |||
# Growth curve for E. coli DB3.1, DH5a and BW27783 did not work as expected. | |||
# (Not recorded) Poured more LB-agar with Janny. All stored in AMBL 4ºC. | |||
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Revision as of 13:33, 7 August 2009
iGEM Project name 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Chloramphenicol Testing Results
Preparation of LB-Agar Plates
Chloramphenicol Testing for Paul's new batch
Growth Curve for E. coli DB3.1, DH5a and BW27783
Steps Taken
Results for OD Readings
Comments on Results
Summary of the Day
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