IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/04: Difference between revisions
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* Spread plate 500uL on Chlor plate | * Spread plate 500uL on Chlor plate | ||
==Results of Chloramphenicol Testing== | |||
* Plates were taken from the Lagally Lab 37ºC incubator at 10:05 am. | |||
* Results: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Plate''' | |||
| align="center" style="background:#f0f0f0;"|'''Condition''' | |||
| align="center" style="background:#f0f0f0;"|'''DB3.1 Growth?''' | |||
| align="center" style="background:#f0f0f0;"|'''DH5a Growth?''' | |||
|- | |||
| Chlor 0||LB-only||Yes||Yes | |||
|- | |||
| Chlor 1||LB + 25µg/mL Chloramphenicol||No||No | |||
|} | |||
===Comments=== | |||
* Obviously, the chloramphenicol works. Now, I will aliquot Paul's batch into 1 mL aliquots | |||
* Discarded old batch of Chlor-25 into biohazardous waste. | |||
* Leftover stock kept in -20ºC freezer | |||
* To help Heather with transformation of Chloramphenicol construction plasmid, I will spread plate 5 chlor plates with 25 µL of Chlor-25 stock on each plate. | |||
Revision as of 13:40, 7 August 2009
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Competence Test for BW27783 cells
Transformation of Chloramphenicol Construction Plasmid
Results of Chloramphenicol Testing
Comments
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