IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/08: Difference between revisions
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* Use 100uL aliquots, store at -80°C?. | * Use 100uL aliquots, store at -80°C?. | ||
==Resuspension and Transformation of BioBricks== | |||
# Worked with Karen and Amelia | |||
# Resuspended BioBricks into 15 µL of sdH2O in new PCR tube | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Part Description''' | |||
| align="center" style="background:#f0f0f0;"|'''Part #''' | |||
| align="center" style="background:#f0f0f0;"|'''Plate/Well''' | |||
|- | |||
| eYFP||E0430||1/8I | |||
|- | |||
| RFP||E1010||1/18F | |||
|- | |||
| J23100 promoter||J23100||1/18C | |||
|- | |||
| RBS||B0034||1/2M | |||
|} | |||
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Revision as of 14:22, 7 August 2009
iGEM Project name 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
Miniprep of Amp Construction Plasmid and Chlor Construction Plasmid
Centrifuge at 10200rpm (8500x g) for 1 min Add 250uL of Wash Buffer 2 and centrifuge at 10200rpm
Results of Miniprep
NOTE: All results were found using a nanodrop with 1uL of sample Making Competent BW27783 and DH5α cells
Resuspension and Transformation of BioBricks
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