IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/11: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 14: | Line 14: | ||
* Used 50uL of Elution Buffer instead of 100uL as wanted as high of a concentration as possible as the number of cells is less then usual (was not inncubated as long as usual) | * Used 50uL of Elution Buffer instead of 100uL as wanted as high of a concentration as possible as the number of cells is less then usual (was not inncubated as long as usual) | ||
* Eric started overnight cultures of all 4 plasmids in case we need to do another miniprep tommorow | * Eric started overnight cultures of all 4 plasmids in case we need to do another miniprep tommorow | ||
==Ampicillin - New stocks testing== | |||
# Made a new stock of Amp-100 | |||
## 20 mL sH2O + 2.0755 g ampicillin sodium salt | |||
## Filter sterilized | |||
# Stored in 2x 15 mL culture tubes (1000X stock) | |||
# Tested by spread plating 20µL of stock | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> |
Revision as of 14:24, 7 August 2009
iGEM Project name 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Miniprep of RBS-RFP-Terminator, Assembly 1 (J23100 + GFP), pBAD reverse and Amp/Tet Construction Plasmid
Ampicillin - New stocks testing
|