IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/06/15: Difference between revisions

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* 2 samples with the negative pBAD plasmid will be miniprepped and then sent for sequencing
* 2 samples with the negative pBAD plasmid will be miniprepped and then sent for sequencing

Revision as of 15:11, 7 August 2009

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pBAD Mutagenesis Results

Plate Colony Count Color
Positive (diluted) 0 N/A
Positive (concentration) 1 N/A
Negative (diluted) TNTC White (all)
Negative (concentration) 135 White (all)
Control (Diluted) 20 blue
1 white
Control (Concentrated) 322 blue
33 white
  • 2 samples with the negative pBAD plasmid will be miniprepped and then sent for sequencing
  • The colony with the positive pBAD plasmid will be miniprepped and then sent for sequencing. As there was only one colony, the plasmid in the cell might be the original plasmid. Hence more PCR experiments will be planned to confirm the validity of the results.


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