IGEM:British Columbia/Protocols/Glycerol Stocks

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  • 60% autoclaved glycerol
  • Day/overnight culture


  • 1.5 microfuge tube/cryovial


  • Label each tube with: strain, date, Biobrick part number/ assembly number, antibiotic and any other identifying information
  • Aseptically add 0.25mL 60% glycerol to the tube, then add 0.75mL of culture (final glycerol concentration 15%).
  • Vortex briefly
  • Store in -80°C freezer
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