IGEM:Brown/2007/Lab Protocols: Difference between revisions

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[[/Creating Competent Cells  -Jamie Gagnon]]
[[/Creating Competent Cells  -Jamie Gagnon]]


[[/M9 Minimal Media | M9 Minimal Media (6/21/07)]]
[[http://openwetware.org/wiki/Endy:M9_medium/minimal | M9 Minimal Media (6/21/07)]]

Revision as of 19:02, 21 June 2007

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/Preparing Antibiotic Stock Solutions

/Pouring Plates 1

/Pouring Plates 2

/Pouring Plates 3

/Autoclave Glassware

/10min E coli Miniprep

/Quick Tform into E. Coli from Qiagen prep DNA -from Brodsky

/Ultra Competent E coli prep - from Brodsky

/Ligation Setup-from Brodsky

/Preparing Ligation Backbone and Insert

/Constructing a Plasmid

/10X Ligation Buffer Recipe

/Creating Competent Cells -Jamie Gagnon

[| M9 Minimal Media (6/21/07)]

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