IGEM:Brown/2007/Lab Protocols/Quick Tform into E. Coli from Qiagen prep DNA -from Brodsky
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Quick Tform into E Coli from Qiagen prep DNA
-Thaw competent Cells -Add 1uL plamid and 5uL competent Cells -sit on ice 10-30 mins -take up cells in 100uL LB -plate on LB/AMP plates -incubate at 37*C.