IGEM:Brown/2007/Sensor: Difference between revisions

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Sensor Work
{{Template:BrowniGEM}}


6/5/07
'''Tutorial
'''


6/5/07
==Calendar==
'''Working with different promoters attached to GFP'''
We'll provide WEEKLY updates on our work on the Lead Sensor. Check the calendar for details.
 
<calendar>
name=iGEM:Brown/2007/Lead-sensor
date=2007/08/01
view=threemonths
format=%name/%year-%month-%day
weekstart=7
</calendar>
 
== Background on the Lead Sensor ==
Public health concern: Lead in soil, paint, water, dust
Lead Poisoning is often caused by ingesting contaminated drinking water, or soil. It can cause neurological and gastrointestinal disorders, especially among children.
 
The legal limit of lead in drinking water is 15 parts per billion.
 
Current ways of testing for lead either require expensive chemical lab analysis or involve inaccurate home kits.
 
So why do we want a biosensor?
- Cheap
- Sensitive
- Quick
- Specific
 
Our system involves a Lead detecting promoter, an amplifier, and an output of GFP. We're also working on a system to remove false positives.
 
 
==Papers and Documents==
 
7/19/07
[[/T7 | T7 Info from Barry Canton (MIT)]]
 
7/17/07
[[/T7 parts list]]
 
6/21/07
 
[[/Draft of sensor design]]
 
[[/AHL tests on Amplifier]]


6/5/07
[[/AHL results on T9002]]
'''Testing for AHL'''


A. biochemical tests:
[[/Using RFP or GFP for sensor]]


AHL, particularly 3-oxo AHL, which is what we use, reacts strongly with Chlorox. if you mix a known concentration of chlorox with an unknown concentration of AHL, and use the DPD assay to determine levels of chlorox over time, you can figure out your initial concentration of AHL. I haven't figured out exactly what the DPD assay is yet, but the article gives the impression that it's relatively quick and easy. I'll look into this a little deeper later today.


http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=92997#B1 (Jeff)
6/13/07
[[/Parts List | Potential Parts List]]


B. biosensor tests:
[[/AHL detection]]


AHL reduction test by Imperial (Tito)
[[/What to detect?]]
http://openwetware.org/wiki/IGEM:IMPERIAL/Protocols/S01656/AHL_reduction_AHL_reduction


6/5/07
'''Tutorial
'''


[[../AHL detection]]
6/5/07
'''Working with different promoters attached to GFP'''


[[../What to detect?]]
6/5/07
'''Testing for AHL'''

Latest revision as of 13:35, 20 July 2007

Home        Contact        Internal        The Team        Lead Sensor        Tri-Stable Switch       



Calendar

We'll provide WEEKLY updates on our work on the Lead Sensor. Check the calendar for details.

<calendar> name=iGEM:Brown/2007/Lead-sensor date=2007/08/01 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Background on the Lead Sensor

Public health concern: Lead in soil, paint, water, dust Lead Poisoning is often caused by ingesting contaminated drinking water, or soil. It can cause neurological and gastrointestinal disorders, especially among children.

The legal limit of lead in drinking water is 15 parts per billion.

Current ways of testing for lead either require expensive chemical lab analysis or involve inaccurate home kits.

So why do we want a biosensor? - Cheap - Sensitive - Quick - Specific

Our system involves a Lead detecting promoter, an amplifier, and an output of GFP. We're also working on a system to remove false positives.


Papers and Documents

7/19/07 T7 Info from Barry Canton (MIT)

7/17/07 /T7 parts list

6/21/07

/Draft of sensor design

/AHL tests on Amplifier

/AHL results on T9002

/Using RFP or GFP for sensor


6/13/07 Potential Parts List

/AHL detection

/What to detect?

6/5/07 Tutorial

6/5/07 Working with different promoters attached to GFP

6/5/07 Testing for AHL

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