IGEM:Brown/2007/Sensor: Difference between revisions
(New page: Sensor Work 6/5/07 Tutorial 6/5/07 Working with different promoters attached to GFP 6/5/07 Testing for AHL A. biochemical tests: AHL, particularly 3-oxo AHL, which is what we use, re...) |
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Working with different promoters attached to GFP | '''Working with different promoters attached to GFP''' | ||
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Testing for AHL | '''Testing for AHL''' | ||
A. biochemical tests: | A. biochemical tests: | ||
Revision as of 09:15, 5 June 2007
Sensor Work
6/5/07 Tutorial
6/5/07 Working with different promoters attached to GFP
6/5/07 Testing for AHL
A. biochemical tests:
AHL, particularly 3-oxo AHL, which is what we use, reacts strongly with Chlorox. if you mix a known concentration of chlorox with an unknown concentration of AHL, and use the DPD assay to determine levels of chlorox over time, you can figure out your initial concentration of AHL. I haven't figured out exactly what the DPD assay is yet, but the article gives the impression that it's relatively quick and easy. I'll look into this a little deeper later today.
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=92997#B1 (Jeff)
B. biosensor tests:
AHL reduction test by Imperial (Tito) http://openwetware.org/wiki/IGEM:IMPERIAL/Protocols/S01656/AHL_reduction_AHL_reduction
