IGEM:Brown/2007/Sensor/AHL results on T9002: Difference between revisions
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(New page: We had a lot of success working with AHL and the T9002 construct. We used Imperial's AHL concentrations as starting points. We used 100 ul of cultured cells in LB combined with: '''1 ul of...) |
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Revision as of 13:59, 21 June 2007
We had a lot of success working with AHL and the T9002 construct. We used Imperial's AHL concentrations as starting points. We used 100 ul of cultured cells in LB combined with: 1 ul of 0 nm AHL solution, 1 nm AHL, 100 nm AHL, and 10,000 AHL.
Our 0 AHL sample was used to show the amount of leakyness within our cell. Some GFP was expressed, about 2000 RFU. (How much does just LB express?)