IGEM:Brown/2008/Notebook/Team Resistance/2008/06/08: Difference between revisions
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==Considerations for gene cassette== | ==Considerations for gene cassette== | ||
Harvard’s paper | Harvard’s paper | ||
• Used luria lb | • Used luria lb | ||
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Materials for lab: | Materials for lab: | ||
More 1000ml wheaton bottles to be able to store lb | More 1000ml wheaton bottles to be able to store lb | ||
(possibly borrow) | (possibly borrow) | ||
Requested meeting with professor palmore | Requested meeting with professor palmore | ||
Questions | Questions | ||
• What kind of things affect resistance (in terms of our apparatus) | • What kind of things affect resistance (in terms of our apparatus) | ||
wires slipped through cardboard | |||
• distance between each wire in plates 0.25 in. and 1.73 in. into plates | • distance between each wire in plates 0.25 in. and 1.73 in. into plates | ||
Revision as of 08:21, 16 June 2008
 iGEM Project name 1
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Team Resistance
Considerations for gene cassetteHarvard’s paper • Used luria lb • Grew cells up to mid-logarithmic stage then added the arabinose • Saw decrease in cell density after just 1 hour, but no change in the control • Used plasmid pvj4 • E.coli strains: DH5alpha Beta2155 • Can we check tightness of promoters by using gfp? Materials for lab: More 1000ml wheaton bottles to be able to store lb (possibly borrow) Requested meeting with professor palmore Questions • What kind of things affect resistance (in terms of our apparatus)
• distance between each wire in plates 0.25 in. and 1.73 in. into plates
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