IGEM:Brown/2008/Notebook/Team Resistance/2008/06/08

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(Autocreate 2008/06/08 Entry for IGEM:Brown/2008/Notebook/Team_Resistance)
(Team Resistance)
Line 8: Line 8:
* Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution.
* Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution.
 +
==Considerations for gene cassette==
 +
Harvard’s paper
 +
• Used luria lb
 +
• Grew cells up to mid-logarithmic stage then added the arabinose
 +
• Saw decrease in cell density after just 1 hour, but no change in the control
 +
• Used plasmid pvj4
 +
• E.coli strains:
 +
o DH5alpha
 +
o Beta2155
 +
• Can we check tightness of promoters by using gfp?
 +
 +
Materials for lab:
 +
More 1000ml wheaton bottles to be able to store lb
 +
(possibly borrow)
 +
 +
Requested meeting with professor palmore
 +
Questions
 +
• What kind of things affect resistance (in terms of our apparatus)
 +
 +
 +
-wires slipped through cardboard
 +
• distance between each wire in plates 0.25 in. and 1.73 in. into plates

Revision as of 11:20, 16 June 2008

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Team Resistance

  • Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution.

Considerations for gene cassette

Harvard’s paper • Used luria lb • Grew cells up to mid-logarithmic stage then added the arabinose • Saw decrease in cell density after just 1 hour, but no change in the control • Used plasmid pvj4 • E.coli strains: o DH5alpha o Beta2155 • Can we check tightness of promoters by using gfp?

Materials for lab: More 1000ml wheaton bottles to be able to store lb (possibly borrow)

Requested meeting with professor palmore Questions • What kind of things affect resistance (in terms of our apparatus)


-wires slipped through cardboard • distance between each wire in plates 0.25 in. and 1.73 in. into plates



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