IGEM:Brown/2008/Notebook/Team Resistance/2008/06/08

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(Team Resistance)
(Considerations for gene cassette)
Line 11: Line 11:
Harvard’s paper
Harvard’s paper
• Used luria lb
• Used luria lb
 +
• Grew cells up to mid-logarithmic stage then added the arabinose
• Grew cells up to mid-logarithmic stage then added the arabinose
 +
• Saw decrease in cell density after just 1 hour, but no change in the control
• Saw decrease in cell density after just 1 hour, but no change in the control
 +
• Used plasmid pvj4
• Used plasmid pvj4
 +
• E.coli strains:
• E.coli strains:
-
o DH5alpha
+
DH5alpha
-
o Beta2155
+
Beta2155
 +
 
• Can we check tightness of promoters by using gfp?
• Can we check tightness of promoters by using gfp?

Revision as of 11:21, 16 June 2008

iGEM Project name 1 Main project page
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Team Resistance

  • Our team is working to create a biosensor that detects the presence of a toxin via the change in resistance of the bacterial solution.

Considerations for gene cassette

Harvard’s paper • Used luria lb

• Grew cells up to mid-logarithmic stage then added the arabinose

• Saw decrease in cell density after just 1 hour, but no change in the control

• Used plasmid pvj4

• E.coli strains: DH5alpha Beta2155

• Can we check tightness of promoters by using gfp?

Materials for lab: More 1000ml wheaton bottles to be able to store lb (possibly borrow)

Requested meeting with professor palmore Questions • What kind of things affect resistance (in terms of our apparatus)


-wires slipped through cardboard • distance between each wire in plates 0.25 in. and 1.73 in. into plates



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