IGEM:Brown/2008/Notebook/Team Resistance/2008/06/08
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Harvard’s paper | Harvard’s paper | ||
• Used luria lb | • Used luria lb | ||
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• Grew cells up to mid-logarithmic stage then added the arabinose | • Grew cells up to mid-logarithmic stage then added the arabinose | ||
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• Saw decrease in cell density after just 1 hour, but no change in the control | • Saw decrease in cell density after just 1 hour, but no change in the control | ||
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• Used plasmid pvj4 | • Used plasmid pvj4 | ||
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• E.coli strains: | • E.coli strains: | ||
| - | + | DH5alpha | |
| - | + | Beta2155 | |
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• Can we check tightness of promoters by using gfp? | • Can we check tightness of promoters by using gfp? | ||
Revision as of 11:21, 16 June 2008
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Team Resistance
Considerations for gene cassetteHarvard’s paper • Used luria lb • Grew cells up to mid-logarithmic stage then added the arabinose • Saw decrease in cell density after just 1 hour, but no change in the control • Used plasmid pvj4 • E.coli strains: DH5alpha Beta2155 • Can we check tightness of promoters by using gfp? Materials for lab: More 1000ml wheaton bottles to be able to store lb (possibly borrow) Requested meeting with professor palmore Questions • What kind of things affect resistance (in terms of our apparatus)
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