IGEM:Brown/2008/Notebook/Team Resistance/2008/06/11
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Team Resistance
Testing apparatus with NaCl at different concentrations • 3 plates with 0.05M NaCl (0.02922g in 10mL) • 3 plates with 0.005M NaCl (0.02922g in 100mL) • 3 plates with 0.001M NaCl (0.01461g in 250mL) dilute to each concentration then put 10 mL of each solution in respective plates Control: distilled water Results: Control: 4.108Mohm, 2.517Mohm, 2.934Mohm 0.001M NaCl: 393.7 kohm, 265.5kohm, 579.6kohm 0.005M NaCl: 264.9kohm, 266kohm, 326.1kohm 0.05M NaCl: 233.5kohm, 46.69kohm, 97.1kohm control 0.001M NaCl 0.005M NaCl 0.05M NaCl 3.186MΩ 0.4129MΩ 0.2857MΩ 0.1258MΩ 0.001M NaCl: 2.7731MΩ change 0.005M NaCl: 2.9003MΩ change 0.05M NaCl: 3.0602MΩ change • Our wires were not long enough to touch the solution in the plates—next time don’t tape wires in place until right before experiment begins • WE NEED A COMPUTER BASED VOLTMETER
Should touching the plastic significantly change the resistance? What if distance changes slightly? All considerations of our apparatus Any suggestions for a real-time, computer based voltmeter? Tomorrow: get plates from Harvard lab! Create glycerol stocks of the plasmids
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