IGEM:Brown/2008/Notebook/Team Resistance/2008/06/17: Difference between revisions
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1) Put 10mL culture (pvj4) into 6, 15mL centrifuge tubes | 1) Put 10mL culture (pvj4) into 6, 15mL centrifuge tubes | ||
Results: | |||
At 600nm | |||
original culture (20 cultures) | |||
==Important Notes== | ==Important Notes== |
Revision as of 09:49, 17 June 2008
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Daily Information
Created different concentrations of cells: 1) Put 10mL culture (pvj4) into 6, 15mL centrifuge tubes
original culture (20 cultures) Important Notes
Used Beckman Centrifuge To balance tubes:
To create bacteria in mid-logarithmic phase
Mid-logarathmic phase: optical density less than 1-- dilute culture in stationary phase (greater than 2 or 3) to 0.05od or 0.1od and then incubate for 45 minutes to an hour to reach mid-logarithminc phase (remember doubling time for E.Coli is once every 20 minutes) To grow cultures, use a culture solution that is 1/4 the volume of the flask Setbacks
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