IGEM:Brown/2008/Notebook/Team Resistance/2008/06/17

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==Daily Information==
==Daily Information==
* insert what you did today here
* insert what you did today here
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Created different concentrations of cells:
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1) Put 10mL culture (pvj4) into 6, 15mL centrifuge tubes
==Important Notes==
==Important Notes==

Revision as of 11:21, 17 June 2008

Image:Team_resistance_log.png Main project page
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Daily Information

  • insert what you did today here

Created different concentrations of cells:

1) Put 10mL culture (pvj4) into 6, 15mL centrifuge tubes

Important Notes

  • insert what was important here

Used Beckman Centrifuge

To balance tubes:

  • Weigh the tubes that will be in one rotor (standing them up in a styrofoam block is easiest)
  • Weigh tubes that will be placed in other rotor
  • If unbalanced, put empty tube in the lighter styrofoam block and add water to tube until both block are the same mass.


  • For bacterial cells, centrifuge for about 10 minutes at a speed a little higher than 1000rpm.

Setbacks

  • Insert any setbacks we had today or lessons to learn from!



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