IGEM:Brown/2008/Notebook/Team Resistance/2008/06/17: Difference between revisions
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*If unbalanced, put empty tube in the lighter styrofoam block and add water to tube until both block are the same mass. | *If unbalanced, put empty tube in the lighter styrofoam block and add water to tube until both block are the same mass. | ||
*For bacterial cells, centrifuge for about 10 minutes at a speed a little higher than 1000rpm. | |||
*Bacteria double every 20 minutes | |||
*An overnight culture (15hrs) should reach stationary phase (od about 10) | |||
*If cell culture density is known (after an overnight culture)-- dilute down to .05 .1 cell density (to make it simple-- can calculate amount of cells after a certain amount of time | |||
*Cells in stationary phase don't grow much -- culture od would probably be out of spec's od range (0.2-0.7) so dilute it down to the linear range and then to 0.05 or 0.1 and let incubate for about an hour | |||
Mid-logarathmic phase: optical density less than 1-- dilute culture in stationary phase (greater than 2 or 3) to 0.05od or 0.1od and then incubate for 45 minutes to an hour to reach mid-logarithminc phase (remember doubling time for E.Coli is once every 20 minutes) | |||
To grow cultures, use a culture solution that is 1/4 the volume of the flask | |||
==Setbacks== | ==Setbacks== |
Revision as of 08:35, 17 June 2008
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Daily Information
Created different concentrations of cells: 1) Put 10mL culture (pvj4) into 6, 15mL centrifuge tubes Important Notes
Used Beckman Centrifuge To balance tubes:
Mid-logarathmic phase: optical density less than 1-- dilute culture in stationary phase (greater than 2 or 3) to 0.05od or 0.1od and then incubate for 45 minutes to an hour to reach mid-logarithminc phase (remember doubling time for E.Coli is once every 20 minutes) To grow cultures, use a culture solution that is 1/4 the volume of the flask Setbacks
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