IGEM:Caltech/2007/Progress: Difference between revisions
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==Week of 2007/06/11== | ==Week of 2007/06/11== | ||
2007/06/13 Wednesday – Transformed the | 2007/06/13 Wednesday – Transformed the BioBricks into bacteria. Titered lambda phage into concentrations of 10, 100, and 1000 colonies per plate.<br> | ||
Used BioBricks:<br> | |||
E0040 GFP | |||
<br> | <br> | ||
2007/06/14 Thursday – Checked results of titered phage (concentration of initial phage sample seemed to be off). Picked one colony from the transformed bacteria and cultured overnight.<br> | 2007/06/14 Thursday – Checked results of titered phage (concentration of initial phage sample seemed to be off). Picked one colony from the transformed bacteria and cultured overnight.<br> | ||
<br> | <br> | ||
2007/06/15 Friday – Purified the plasmids from the transformed bacteria colony using a prep kit, added restriction enzymes and buffer solution. Plan to integrate the terminator, constitutive promoter, and the tetR gene system (RBS, tetR gene, and terminator) | 2007/06/15 Friday – Purified the plasmids from the transformed bacteria colony using a prep kit, added restriction enzymes and buffer solution. Plan to integrate the terminator, constitutive promoter, and the tetR gene system (RBS, tetR gene, and terminator) BioBrick parts onto the 2K3 plasmid.<br> | ||
Part name: Enzymes used: Buffer:<br> | |||
Revision as of 22:23, 17 June 2007
Week of 2007/06/11
2007/06/13 Wednesday – Transformed the BioBricks into bacteria. Titered lambda phage into concentrations of 10, 100, and 1000 colonies per plate.
Used BioBricks:
E0040 GFP
2007/06/14 Thursday – Checked results of titered phage (concentration of initial phage sample seemed to be off). Picked one colony from the transformed bacteria and cultured overnight.
2007/06/15 Friday – Purified the plasmids from the transformed bacteria colony using a prep kit, added restriction enzymes and buffer solution. Plan to integrate the terminator, constitutive promoter, and the tetR gene system (RBS, tetR gene, and terminator) BioBrick parts onto the 2K3 plasmid.
Part name: Enzymes used: Buffer:
