IGEM:Caltech/2007/Progress: Difference between revisions
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E0040 GFP<br> | E0040 GFP<br> | ||
constitutive promoter<br> | constitutive promoter<br> | ||
terminator<br> | B0015 terminator<br> | ||
tetR gene<br> | P0440 tetR gene<br> | ||
2K3 plasmid<br> | 2K3 plasmid<br> | ||
<br> | <br> | ||
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2007/06/15 Friday – Purified the plasmids from the transformed bacteria colony using a prep kit, added restriction enzymes and buffer solution. Plan to integrate the terminator, constitutive promoter, and the tetR gene system (RBS, tetR gene, and terminator) BioBrick parts onto the 2K3 plasmid.<br> | 2007/06/15 Friday – Purified the plasmids from the transformed bacteria colony using a prep kit, added restriction enzymes and buffer solution. Plan to integrate the terminator, constitutive promoter, and the tetR gene system (RBS, tetR gene, and terminator) BioBrick parts onto the 2K3 plasmid.<br> | ||
Part name: Enzymes used: Buffer:<br> | Part name: Enzymes used: Buffer:<br> | ||
P0440 Xba/Pst Eco Buffer & BSA <br> | |||
B0015 Eco/Pst Eco Buffer & BSA <br> | |||
2K3 plasmid Eco/Pst Eco Buffer & BSA <br> | |||
constitutive promoter Spe/Pst NEB & Buffer 2 | |||
Revision as of 22:54, 17 June 2007
Week of 2007/06/11
2007/06/13 Wednesday – Transformed the BioBricks into bacteria. Titered lambda phage into concentrations of 10, 100, and 1000 colonies per plate.
Used BioBricks:
E0040 GFP
constitutive promoter
B0015 terminator
P0440 tetR gene
2K3 plasmid
2007/06/14 Thursday – Checked results of titered phage (concentration of initial phage sample seemed to be off). Picked one colony from the transformed bacteria and cultured overnight.
2007/06/15 Friday – Purified the plasmids from the transformed bacteria colony using a prep kit, added restriction enzymes and buffer solution. Plan to integrate the terminator, constitutive promoter, and the tetR gene system (RBS, tetR gene, and terminator) BioBrick parts onto the 2K3 plasmid.
Part name: Enzymes used: Buffer:
P0440 Xba/Pst Eco Buffer & BSA
B0015 Eco/Pst Eco Buffer & BSA
2K3 plasmid Eco/Pst Eco Buffer & BSA
constitutive promoter Spe/Pst NEB & Buffer 2
