IGEM:Caltech/2007/Project: Difference between revisions
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The goal of our project is to create a λ bacteriophage that can selectively lyse specific subpopulations of ''E. coli''. We will first use recombineering techniques to insert amber mutations into three key developmental genes in λ-Zap. Next, a second copy of these genes, controlled by a cis-repressing riboregulator, will be cloned into the phage genome. The expression of trans-activating RNA in the target bacterial host will relieve the repression, complement the engineered phage, and allow lysis. Hosts which do not contain this RNA will remain intact. | The goal of our project is to create a λ bacteriophage that can selectively lyse specific subpopulations of ''E. coli''. We will first use recombineering techniques to insert amber mutations into three key developmental genes in λ-Zap. Next, a second copy of these genes, controlled by a cis-repressing riboregulator, will be cloned into the phage genome. The expression of trans-activating RNA in the target bacterial host will relieve the repression, complement the engineered phage, and allow lysis. Hosts which do not contain this RNA will remain intact. | ||
[[Image:Caltech_2007_overview.gif|center]] | |||
==<center>Project Details</center>== | ==<center>Project Details</center>== |
Revision as of 16:48, 24 October 2007
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