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The Caltech iGEM 2007 team is composed of four
The Caltech iGEM 2007 team is composed of four undergraduates and one undergraduate from MIT. Team members are current juniors and seniors in biology, chemistry, chemical engineering, and biological engineering. The team was advised by three graduate students and three faculty mentors.
to λ bacteriophage lyse specific subpopulations of ''E. coli'' . We will first use recombineering techniques to insert amber mutations into three key developmental genes in λ-Zap. Next, a second copy of these genes, controlled by a cis-repressing riboregulator, will be cloned into the phage genome at the ribosome binding site upstream of each of the three critical genes, thus blocking the expression of key viral developmental proteins. As depicted in the diagram below, the expression of trans-activating RNA in the target bacterial host will relieve the repression by opening up the ribosome binding site, enable the translation of the viral developmental gene and allow lysis of the host cell. Hosts which do not contain this RNA will remain intact.