IGEM:Caltech/2007/Project: Difference between revisions
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The goal of our project is to create a λ bacteriophage that can selectively lyse specific subpopulations of ''E. coli''. We will first use recombineering techniques to insert amber mutations into three key developmental genes in λ-Zap. Next, a second copy of these genes, controlled by a cis-repressing riboregulator, will be cloned into the phage genome. | The goal of our project is to create a λ bacteriophage that can selectively lyse specific subpopulations of ''E. coli''. We will first use recombineering techniques to insert amber mutations into three key developmental genes in λ-Zap. Next, a second copy of these genes, controlled by a cis-repressing riboregulator, will be cloned into the phage genome at the ribosome binding site upstream of each of the three critical genes, thus blocking the expression of key viral developmental proteins. As depicted in the diagram below, the expression of trans-activating RNA in the target bacterial host will relieve the repression by opening up the ribosome binding site, enable the translation of the viral developmental gene and allow lysis of the host cell. Hosts which do not contain this RNA will remain intact. | ||
[[Image:Caltech_2007_overview.gif|center]] | [[Image:Caltech_2007_overview.gif|center]] |
Revision as of 12:34, 25 October 2007
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