IGEM:Caltech/2007/Project: Difference between revisions
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* [[IGEM:Caltech/2007/Project/construct|Explanation of Construct]] | * [[IGEM:Caltech/2007/Project/construct|Explanation of Construct]] | ||
* [[IGEM:Caltech/2007/Project/constructList|List of Cloned Constructs]] | * [[IGEM:Caltech/2007/Project/constructList|List of Cloned Constructs]] | ||
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Titering experiments where cro, N, and Q amber phages were allowed to infect D1210 cells containing the built construct show that heterologous N and Q can complement phages with amber mutations in the respective genes. Adding a cis-repressor to the Q construct lowered production of Q even further, as it eliminated lysis completely. We were unable to express sufficient cro from a plasmid to rescue a cro mutant phage. | Titering experiments where cro, N, and Q amber phages were allowed to infect D1210 cells containing the built construct show that heterologous N and Q can complement phages with amber mutations in the respective genes. Adding a cis-repressor to the Q construct lowered production of Q even further, as it eliminated lysis completely. We were unable to express sufficient cro from a plasmid to rescue a cro mutant phage. |
Revision as of 08:11, 26 October 2007
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