N Antiterminator
Background Information
What is N?
N protein is one of the three critical proteins expressed by the lambda genome that influence the developmental cycles of the virus. It is called an antiterminator because in the presence of this protein, RNA polymerase is able to code through regions of the genome it would otherwise be unable to transcribe. As depicted in the diagram below, there is a termination sequence early in the lambda genome, which causes the mRNA transcript to terminate in the absence of N. However, when N is present, the protein recognizes a specific sequence called Nut, which stands for N utilization, and as the polymerase passes over the Nut site, it is modified by N so that it can ignore the termination sequence that is downstream of the Nut site, thus allowing the elongation of the mRNA transcript.
Role in Lambda Life Cycle
What does N do?
Original Goals
What were you trying to do? Also mention the liquid assays
Status and Future Plans
What did you end up doing, and what's next?
Relevant Protocols
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