IGEM:Caltech/2007/Project/Q: Difference between revisions
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Once Q has modified the RNA polymerase, the polymerase begins to transcribe genes beyond tR'. These genes are crucial to the production of new phage components, such as tails and heads (protein capsules). Once these genes are expressed, the phage is committed to the lytic pathway. | Once Q has modified the RNA polymerase, the polymerase begins to transcribe genes beyond tR'. These genes are crucial to the production of new phage components, such as tails and heads (protein capsules). Once these genes are expressed, the phage is committed to the lytic pathway. | ||
[[Image:Bacteriophage_lambda_genome.png|thumb|Schematic representation of the genome of the bacteriophage lambda. Q alters RNA polymerase and allows transcription of genes coding for heads and tails production.]] | [[Image:Bacteriophage_lambda_genome.png|thumb|center|Schematic representation of the genome of the bacteriophage lambda. Q alters RNA polymerase and allows transcription of genes coding for heads and tails production.]] | ||
[[Image:Q_changes.jpg|center|changes brought on by the Q protein]] | [[Image:Q_changes.jpg|center|changes brought on by the Q protein]] | ||
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[[Image:Q_results.jpg|center|Titering results for Q construct without cis repressor]] | [[Image:Q_results.jpg|center|Titering results for Q construct without cis repressor]] | ||
Future work on Q includes titering the construct featuring the strong constitutive promoter, introducing the conjugate trans activators to Q construct variants containing cis repressors, and quantitatively measuring the effects of aTc concentration on cell lysis rates. | Future work on Q includes titering the construct featuring the strong constitutive promoter, introducing the conjugate trans activators to Q construct variants containing cis repressors, and quantitatively measuring the effects of aTc concentration on cell lysis rates. |
Latest revision as of 00:18, 26 October 2007
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