IGEM:Caltech/2008/Project/Lactose intolerance: Difference between revisions
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(New page: =Lactose Summary= We first want to make two plasmids: Plasmid #1 - A constitutive promoter with <i>LacZ</i> and <i>LacY</i>. Plasmid #2 - A lactose inducible promoter with lysozyme and hol...) |
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=Lactose Summary= | =Lactose Summary= | ||
We first want to make two plasmids: | We first want to make two plasmids:<br> | ||
Plasmid #1 - A constitutive promoter with <i>LacZ</i> and <i>LacY</i>. | Plasmid #1 - A constitutive promoter with <i>LacZ</i> and <i>LacY</i>.<br> | ||
Plasmid #2 - A lactose inducible promoter with lysozyme and holin. | Plasmid #2 - A lactose inducible promoter with lysozyme and holin.<br> | ||
The general idea is to overexpress LacY (Lactose receptor protein) with LacZ (Beta-Galactosidase). When lactose is present, the cell will uptake lactose and will flip on the LacI / LacIQ promoter when enough lactose is present. We then express lysozyme and holin which will lyse the cell releasing beta-gal cleaving lactose into glucose and galactose. Hopefully, glucose and galactose will be taken up by the host in the colon. | The general idea is to overexpress LacY (Lactose receptor protein) with LacZ (Beta-Galactosidase). When lactose is present, the cell will uptake lactose and will flip on the LacI / LacIQ promoter when enough lactose is present. We then express lysozyme and holin which will lyse the cell releasing beta-gal cleaving lactose into glucose and galactose. Hopefully, glucose and galactose will be taken up by the host in the colon. | ||
==Lactose Outline== | ==Lactose Outline== | ||
Revision as of 14:43, 19 June 2008
Lactose Summary
We first want to make two plasmids:
Plasmid #1 - A constitutive promoter with LacZ and LacY.
Plasmid #2 - A lactose inducible promoter with lysozyme and holin.
The general idea is to overexpress LacY (Lactose receptor protein) with LacZ (Beta-Galactosidase). When lactose is present, the cell will uptake lactose and will flip on the LacI / LacIQ promoter when enough lactose is present. We then express lysozyme and holin which will lyse the cell releasing beta-gal cleaving lactose into glucose and galactose. Hopefully, glucose and galactose will be taken up by the host in the colon.
