IGEM:Caltech/2008/Project/Lactose intolerance: Difference between revisions
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<b>6.20.08</b><br> | <b>6.20.08</b><br> | ||
Colonies grew from I732017 LacZ transformation. They will be cultured Sunday afternoon.<br> | Colonies grew from I732017 LacZ transformation. They will be cultured Sunday afternoon.<br> | ||
LacY, LacY +H, and LacY +2H has been inputted into the registry of parts. Primers for LacY site directed mutagenesis were designed.<br>When primers for LacY arrive, need to PCR LacY out of E. coli genomic DNA. | LacY, LacY +H, and LacY +2H has been inputted into the registry of parts. Primers for LacY site directed mutagenesis were designed.<br> | ||
When primers for LacY arrive, need to PCR LacY out of E. coli genomic DNA.<br> | |||
After LacZ culture is grown, mini prep DNA. | |||
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</div> | </div> |
Revision as of 18:41, 21 June 2008
Lactose SummaryWe want to make two plasmids: Lactose Outline6.19.08 |