IGEM:Caltech/2008/Project/Lactose intolerance: Difference between revisions
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We have decided to use the I732017 LacZ (1009/11H) because we could not transform I732005 LacZ (1018/6F).<br> | We have decided to use the I732017 LacZ (1009/11H) because we could not transform I732005 LacZ (1018/6F).<br> | ||
To double check, we will plate our transformed I732005 LacZ with 100μL onto a kan plate, and the rest on an Amp plate.<br> | To double check, we will plate our transformed I732005 LacZ with 100μL onto a kan plate, and the rest on an Amp plate.<br> | ||
We ordered primers for LacY, and we will need to PCR out LacY. We then need to run | We ordered primers for LacY, and we will need to PCR out LacY. We then need to run site directed mutagenesis on LacY by added 2H to prevent EIIA(GLUC) binding.<br> | ||
For our constitutive promoter, we will use various strengths to test our beta-gal concentrations.<br> | For our constitutive promoter, we will use various strengths to test our beta-gal concentrations.<br> | ||
Low expression - J23113<br> | Low expression - J23113<br> |
Revision as of 22:20, 19 June 2008
Lactose SummaryWe want to make two plasmids: Lactose Outline6.19.08 |