IGEM:Caltech/2008/Project/Lactose intolerance: Difference between revisions
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LacY, LacY +H, and LacY +2H has been inputted into the registry of parts. Primers for LacY site directed mutagenesis were designed.<br> | LacY, LacY +H, and LacY +2H has been inputted into the registry of parts. Primers for LacY site directed mutagenesis were designed.<br> | ||
When primers for LacY arrive, need to PCR LacY out of E. coli genomic DNA.<br> | When primers for LacY arrive, need to PCR LacY out of E. coli genomic DNA.<br> | ||
After LacZ culture is grown, mini prep DNA. | After LacZ culture is grown, mini prep DNA.<br> | ||
<b>6.21.08</b><br> | |||
Set up o/n cultures.<br> | |||
1. LacZ in Amp<br> | |||
2. pSB2K3 in Km<br> | |||
3. p1005 in Amp<br> | |||
4. p1005 in Amp + Tet (Just to test the strain is both Tet and Amp resistance) | |||
|} | |} | ||
</div> | </div> |
Revision as of 17:18, 22 June 2008
Lactose SummaryWe want to make two plasmids: Lactose Outline6.19.08 |