IGEM:Caltech/2008/Project/ROS

---

iGEM 2008

Home        People        Project        Protocols        Notes        Ideas        Changes       

---

Conjugation Can get conjugation from Salmonella to E. coli [1] Papers seem to assume that conjugation is quick, and maintenance is the hard part (unless we engineer a shuttle vector). Doesn't address specificity, though. Doug Tischer 21:12, 26 May 2008 (EDT)I'm not concerned about how well the recipient mantains the plasmid. In fact that could be a very good thing. If we use the approach where we spare every good bacteria by giving them a copy of the plasmid, then it would be VERY bad if even one of the cells of infectious bacteria got the plasmid, as they would be subsequently immune from attack. "Conjugation of S. typhi with E. coli F+ carrying P1CM+ gave three types of S. typhi CMr clones: those which carry the whole P1CMphage, those with the PldCM element, and those with nontransferable CMr."[2] Can get transfer of an F' plasmid from E. coli to Salmonella[3] Doug Tischer 21:12, 26 May 2008 (EDT)There exists a plasmid (pAT187) that can be transfered from E. coli to a wide range of gram-positive bacteria [4] Specificity Phage tailspike proteins[5] are specific for Salmonella. References Makanera A, Arlet G, Gautier V, and Manai M. Molecular epidemiology and characterization of plasmid-encoded beta-lactamases produced by Tunisian clinical isolates of Salmonella enterica serotype Mbandaka resistant to broad-spectrum cephalosporins. J Clin Microbiol. 2003 Jul;41(7):2940-5. DOI:10.1128/JCM.41.7.2940-2945.2003 | Kondo E and Mitsuhashi S. Drug resistance of enteric bacteria. VI. Introduction of bacteriophage P1CM into Salmonella typhi and formation of PldCM and F-CM elements. J Bacteriol. 1966 May;91(5):1787-94. DOI:10.1128/jb.91.5.1787-1794.1966 | Lenny AB and Margolin P. Locations of the opp and supX genes of Salmonella typhimurium and Escherichia coli. J Bacteriol. 1980 Aug;143(2):747-52. DOI:10.1128/jb.143.2.747-752.1980 | Mazodier P, Petter R, and Thompson C. Intergeneric conjugation between Escherichia coli and Streptomyces species. J Bacteriol. 1989 Jun;171(6):3583-5. DOI:10.1128/jb.171.6.3583-3585.1989 | Salgado CJ, Zayas M, and Villafane R. Homology between two different Salmonella phages: Salmonella enterica serovar Typhimurium phage P22 and Salmonella enterica serovar Anatum var. 15 + phageepsilon34. Virus Genes. 2004 Aug;29(1):87-98. DOI:10.1023/B:VIRU.0000032792.86188.fb | Plainkum P, Fuchs SM, Wiyakrutta S, and Raines RT. Creation of a zymogen. Nat Struct Biol. 2003 Feb;10(2):115-9. DOI:10.1038/nsb884 | Firbank SJ, Rogers MS, Wilmot CM, Dooley DM, Halcrow MA, Knowles PF, McPherson MJ, and Phillips SE. Crystal structure of the precursor of galactose oxidase: an unusual self-processing enzyme. Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):12932-7. DOI:10.1073/pnas.231463798 | Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng. 2001 Sep;14(9):699-704. DOI:10.1093/protein/14.9.699 | Uehara Y, Kikuchi K, Nakamura T, Nakama H, Agematsu K, Kawakami Y, Maruchi N, and Totsuka K. H(2)O(2) produced by viridans group streptococci may contribute to inhibition of methicillin-resistant Staphylococcus aureus colonization of oral cavities in newborns. Clin Infect Dis. 2001 May 15;32(10):1408-13. DOI:10.1086/320179 | Toomey D and Mayhew SG. Purification and characterisation of NADH oxidase from Thermus aquaticus YT-1 and evidence that it functions in a peroxide-reduction system. Eur J Biochem. 1998 Feb 1;251(3):935-45. DOI:10.1046/j.1432-1327.1998.2510935.x | Whittaker JW. The radical chemistry of galactose oxidase. Arch Biochem Biophys. 2005 Jan 1;433(1):227-39. DOI:10.1016/j.abb.2004.08.034 |