IGEM:Caltech/2008/Project/ROS: Difference between revisions
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*Can get conjugation from Salmonella to E. coli <cite>makanera</cite> | *Can get conjugation from Salmonella to E. coli <cite>makanera</cite> | ||
**Papers seem to assume that conjugation is quick, and maintenance is the hard part (unless we engineer a shuttle vector). Doesn't address specificity, though. | **Papers seem to assume that conjugation is quick, and maintenance is the hard part (unless we engineer a shuttle vector). Doesn't address specificity, though. | ||
*"Conjugation of S. typhi with E. coli F+ carrying P1CM+ gave three types of S. typhi CMr clones: those which carry the whole P1CMphage, those with the PldCM element, and those with nontransferable CMr."<cite>kondo</cite> | *"Conjugation of S. typhi with E. coli F+ carrying P1CM+ gave three types of S. typhi CMr clones: those which carry the whole P1CMphage, those with the PldCM element, and those with nontransferable CMr."<cite>kondo</cite> | ||
*Can get transfer of an F' plasmid from E. coli to Salmonella<cite>lenny</cite> | *Can get transfer of an F' plasmid from E. coli to Salmonella<cite>lenny</cite> | ||
*[[User:Doug Tischer|Doug Tischer]] 21:12, 26 May 2008 (EDT)There exists a plasmid (pAT187) that can be transfered from E. coli to a wide range of gram-positive bacteria <cite>Mazodier</cite> | *[[User:Doug Tischer|Doug Tischer]] 21:12, 26 May 2008 (EDT)There exists a plasmid (pAT187) that can be transfered from E. coli to a wide range of gram-positive bacteria <cite>Mazodier</cite> | ||
*[[User:Doug Tischer|Doug Tischer]] 17:42, 27 May 2008 (EDT)Surface exclusion seems to be the name given to the process that prevents a bacteria containing a plasmid from conjugating and receiving the same plasmid again. In F plasmid, the are the ''traS'' and ''traT'' genes. ''traT'' inhibits the formation of mating aggregates. <cite>Achtman</cite> | |||
==Specificity== | ==Specificity== |