IGEM:Caltech/2008/Project/ROS: Difference between revisions
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*[[User:Doug Tischer|Doug Tischer]] 21:12, 26 May 2008 (EDT)There exists a plasmid (pAT187) that can be transfered from E. coli to a wide range of gram-positive bacteria <cite>Mazodier</cite> | *[[User:Doug Tischer|Doug Tischer]] 21:12, 26 May 2008 (EDT)There exists a plasmid (pAT187) that can be transfered from E. coli to a wide range of gram-positive bacteria <cite>Mazodier</cite> | ||
*[[User:Doug Tischer|Doug Tischer]] 17:42, 27 May 2008 (EDT)Surface exclusion seems to be the name given to the process that prevents a bacteria containing a plasmid from conjugating and receiving the same plasmid again. In F plasmid, the are the ''traS'' and ''traT'' genes. ''traT'' inhibits the formation of mating aggregates. <cite>Achtman</cite> | *[[User:Doug Tischer|Doug Tischer]] 17:42, 27 May 2008 (EDT)Surface exclusion seems to be the name given to the process that prevents a bacteria containing a plasmid from conjugating and receiving the same plasmid again. In F plasmid, the are the ''traS'' and ''traT'' genes. ''traT'' inhibits the formation of mating aggregates. <cite>Achtman</cite> | ||
*[[User:Doug Tischer|Doug Tischer]] 17:51, 27 May 2008 (EDT)Biobrick part [http://partsregistry.org/wiki/index.php/Part:BBa_J01000 BBa_J01000 (TraJf)] is a transcriptional activator that turns on the expression of sex pili forming genes off of the F plasmid. This could be used to trigger the oxidative burst upon conjugation instead. | |||
==Specificity== | ==Specificity== |