IGEM:Caltech/2008/Project/Vitamins

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(References)
Line 12: Line 12:
===Background Information on Folate===
===Background Information on Folate===
    
    
-
Folate, the generic term for the various forms of Vitamin B9, is an essential vitamin because it is heavily involved in amino acid synthesis as well as single-carbon transfer reactions. Folate deficiencies in women can result in birth defects such as neural tube defects and other spinal cord malformations. As important as folate is, humans are unable to produce folate, and so must obtain it from eating foods such as green leafy vegetables or folate-fortified cereals (W. Sybesma 2003). An engineered strain of bacteria that would constantly release folate into the gut would reduce the need to fortify breads and cereals with folate, as well as reduce folate-related birth defects in regions with little access to folate-containing foods. In addition to all the reasons stated above, folate is an ideal vitamin to be produced in the gut because it has been shown to be absorbed in physiologically relevant quantities in the large intestine (Asrar and O’Connor 2005). This is not the case for many vitamins (Asrar and O’Connor 2005).
+
Folate, the generic term for the various forms of Vitamin B9, is an essential vitamin because it is heavily involved in amino acid synthesis as well as single-carbon transfer reactions. Folate deficiencies in women can result in birth defects such as neural tube defects and other spinal cord malformations. As important as folate is, humans are unable to produce folate, and so must obtain it from eating foods such as green leafy vegetables or folate-fortified cereals <cite>sybesma1</cite>. An engineered strain of bacteria that would constantly release folate into the gut would reduce the need to fortify breads and cereals with folate, as well as reduce folate-related birth defects in regions with little access to folate-containing foods. In addition to all the reasons stated above, folate is an ideal vitamin to be produced in the gut because, unlike many other vitamins, it has been shown to be absorbed in physiologically relevant quantities in the large intestine<cite>asrar</cite>.
===Why Folate?===
===Why Folate?===
Line 21: Line 21:
==References==
==References==
<biblio>
<biblio>
-
 
+
#bernstein pmid=18396082
-
<\biblio>
+
#camilo pmid=8613033
 +
#asrar pmid=16081276
 +
#bermingham pmid=12111724
 +
#gabelli pmid=17698004
 +
#sybesma1 pmid=15113564
 +
#sybesma2 pmid=12788700
 +
#sheng pmid=16885287
 +
#morita pmid=11386882
 +
#yun pmid=18051328
 +
#zhu pmid=16269750
 +
#wegkamp1 pmid=15128580
 +
#wegkamp2 pmid=17308179
 +
</biblio>
|}
|}

Revision as of 14:25, 7 July 2008


iGEM 2008

Home        People        Project        Protocols        Notes        Ideas        Changes       


Contents

Vitamin production

Background Information on Folate

Folate, the generic term for the various forms of Vitamin B9, is an essential vitamin because it is heavily involved in amino acid synthesis as well as single-carbon transfer reactions. Folate deficiencies in women can result in birth defects such as neural tube defects and other spinal cord malformations. As important as folate is, humans are unable to produce folate, and so must obtain it from eating foods such as green leafy vegetables or folate-fortified cereals [1]. An engineered strain of bacteria that would constantly release folate into the gut would reduce the need to fortify breads and cereals with folate, as well as reduce folate-related birth defects in regions with little access to folate-containing foods. In addition to all the reasons stated above, folate is an ideal vitamin to be produced in the gut because, unlike many other vitamins, it has been shown to be absorbed in physiologically relevant quantities in the large intestine[2].

Why Folate?

Folate Biosynthesis Pathway

System Design

Folate Detection Methods

References

  1. Sybesma W, Burgess C, Starrenburg M, van Sinderen D, and Hugenholtz J. . pmid:15113564. PubMed HubMed [sybesma1]
  2. Asrar FM and O'Connor DL. . pmid:16081276. PubMed HubMed [asrar]
  3. Bernstein JR, Bulter T, and Liao JC. . pmid:18396082. PubMed HubMed [bernstein]
  4. Camilo E, Zimmerman J, Mason JB, Golner B, Russell R, Selhub J, and Rosenberg IH. . pmid:8613033. PubMed HubMed [camilo]
  5. Bermingham A and Derrick JP. . pmid:12111724. PubMed HubMed [bermingham]
  6. Gabelli SB, Bianchet MA, Xu W, Dunn CA, Niu ZD, Amzel LM, and Bessman MJ. . pmid:17698004. PubMed HubMed [gabelli]
  7. Sybesma W, Starrenburg M, Kleerebezem M, Mierau I, de Vos WM, and Hugenholtz J. . pmid:12788700. PubMed HubMed [sybesma2]
  8. Sheng H, Knecht HJ, Kudva IT, and Hovde CJ. . pmid:16885287. PubMed HubMed [sheng]
  9. Morita M, Asami K, Tanji Y, and Unno H. . pmid:11386882. PubMed HubMed [morita]
  10. Yun J, Park J, Park N, Kang S, and Ryu S. . pmid:18051328. PubMed HubMed [yun]
  11. Zhu T, Pan Z, Domagalski N, Koepsel R, Ataai MM, and Domach MM. . pmid:16269750. PubMed HubMed [zhu]
  12. Wegkamp A, Starrenburg M, de Vos WM, Hugenholtz J, and Sybesma W. . pmid:15128580. PubMed HubMed [wegkamp1]
  13. Wegkamp A, van Oorschot W, de Vos WM, and Smid EJ. . pmid:17308179. PubMed HubMed [wegkamp2]
All Medline abstracts: PubMed HubMed
Personal tools