IGEM:Cambridge/2008/Electric Output

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Aim

To create a system which responds to ligand binding with a detectable voltage caused by a K⁺ flux.

Background

Presentation

Current Progress

-Ordered mutant E.coli chassis to test: Kch^-, KdpD^-, KdpE^-, KdpF-E^-

-Designed two plasmids:

--Kdp Plasmid

--Media:Kdp Plasmid

--Media:Glu Gated channel Plasmid

-Sourced V.harveyi BB170 culture to obtain glutamate-gated potassium channel gene

-Modelled protein structure

-Extracted registry parts to use in plasmids: --Promoter --RBS --Terminator

-Decided on oxygen electrode for small voltage change measurements

Useful Links

Protein prediction tools

Uniprot database

Literature

Kdp operon diagram

Kdp plasmid

The Kdp-ATPase system and its regulation

Potential Chassis: Strain JW1242-1 Strain JW0710-1

Kdp mutant - paper from 1971

Worldwide E.coli Databases

Characterisation of kdpD - 2005