IGEM:Cambridge/2008/Electric Output: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 13: | Line 13: | ||
-Designed two plasmids: | -Designed two plasmids: | ||
* [[Kdp Plasmid]] | |||
* [[Glu Gated channel Plasmid]] | |||
-Sourced V.harveyi BB170 culture to obtain glutamate-gated potassium channel gene | -Sourced V.harveyi BB170 culture to obtain glutamate-gated potassium channel gene | ||
| Line 21: | Line 21: | ||
-Modelled protein structure | -Modelled protein structure | ||
-Extracted registry | -Designed primers for extraction of Kdp Operon from E.coli and Glu Gated channel gene from V.harveyi | ||
-Extracted BioBricks from registry to use in plasmids: | |||
* Promoter BBa_J23100 | |||
* RBS BBa_B0030 | |||
* Terminators BBa_B1002 and BBa_B1006 | |||
-Decided on oxygen electrode for small voltage change measurements | -Decided on oxygen electrode for small voltage change measurements | ||
Revision as of 08:27, 17 July 2008
Aim
To create a system which responds to ligand binding with a detectable voltage caused by a K+ flux.
Background
Current Progress
-Ordered mutant E.coli chassis to test: Kch-, KdpD-, KdpE-, KdpF-E-
-Designed two plasmids:
-Sourced V.harveyi BB170 culture to obtain glutamate-gated potassium channel gene
-Modelled protein structure
-Designed primers for extraction of Kdp Operon from E.coli and Glu Gated channel gene from V.harveyi
-Extracted BioBricks from registry to use in plasmids:
- Promoter BBa_J23100
- RBS BBa_B0030
- Terminators BBa_B1002 and BBa_B1006
-Decided on oxygen electrode for small voltage change measurements
Useful Links
Literature
The Kdp-ATPase system and its regulation
Potential Chassis: Strain JW1242-1 Strain JW0710-1
